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Anti-IEX1/IER3 Antibody (A12282)

Western Blot - Anti-IEX1/IER3 Antibody (A12282) - Antibodies.com Immunohistochemistry - Anti-IEX1/IER3 Antibody (A12282) - Antibodies.com Immunohistochemistry - Anti-IEX1/IER3 Antibody (A12282) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to IEX1/IER3 for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-IEX1/IER3 Antibody
Description
Rabbit polyclonal antibody to IEX1/IER3.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human IER3 (NP_003888.2).
Sequence
GRPSASRGHRKRSRRVLYPRVVRRQLPVEEPNPAKRLLFLLLTIVFCQILMAEEGVPAPLPPEDAPNAASLAPTPVSAVLEPFNLTSEPSDYALDLSTFLQQHPAAF
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
17 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-IEX1/IER3 Antibody (A12282) - Antibodies.comEnlarge Image

Western Blot - Anti-IEX1/IER3 Antibody (A12282)

Western blot analysis of extracts of various cell lines, using Anti-IEX1/IER3 Antibody (A12282) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-IEX1/IER3 Antibody (A12282) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-IEX1/IER3 Antibody (A12282)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-IEX1/IER3 Antibody (A12282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-IEX1/IER3 Antibody (A12282) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-IEX1/IER3 Antibody (A12282)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-IEX1/IER3 Antibody (A12282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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