IGFBP6 expression in Human Adrenal Gland lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-IGFBP6 Antibody (A84511) at 0.5µg/ml and detected by chemiluminescence.
IGFBP6 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress IGFBP6 and incubated with Anti-IGFBP6 Antibody (A84511). Control: Mock-transfected HEK293 cell lysate incubated with Anti-IGFBP6 Antibody (A84511) (first lane).
IGFBP6 expression in Human Skeletal Muscle analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-IGFBP6 Antibody (A84511) at 5µg/ml and revealed with alkaline phosphatase (AP).
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