Rabbit polyclonal antibody to IkappaB- beta (Phospho-Thr19)
Specificity
The antibody detects endogenous level of I kappaB- beta only when phosphorylated at Threonine 19.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Threonine 19 (C-D-S(p)-G-L) derived from Human IkappaB- beta.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Mouse brain tissue using IkappaB- beta (Phospho-Thr19) Antibody #12011.The lane on the left is treated with the antigen-specific peptide.
