Anti-IkappaB-epsilon (phospho Ser22) Antibody (A93583)

Name: Anti-IkappaB-epsilon (phospho Ser22) Antibody
Brand: Antibodies.com
SKU: A93583
Price: 115.0 GBP
Availability: InStock

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$190

Rabbit polyclonal antibody to IkappaB-epsilon (phospho Ser22) for WB, IHC, IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-IkappaB-epsilon (phospho Ser22) Antibody

Description

Rabbit polyclonal antibody to IkappaB-epsilon (phospho Ser22).

Specificity

This antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at Ser22.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:1000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22 (amino acids 131-180).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

38kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

I-kappa-B-epsilon, IkappaBepsilon, IkB-E, IkB-epsilon, IKBE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE, NFKBIE

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-IkappaB-epsilon (phospho Ser22) Antibody (A93583)

Western blot analysis of lysates from Jurkat cells treated with TNF-a 20ng/ml 30' using Anti-IkappaB-epsilon (phospho Ser22) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunohistochemistry - Anti-IkappaB-epsilon (phospho Ser22) Antibody (A93583)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-IkappaB-epsilon (phospho Ser22) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Immunofluorescence - Anti-IkappaB-epsilon (phospho Ser22) Antibody (A93583)

Immunofluorescence analysis of HeLa cells using Anti-IkappaB-epsilon (phospho Ser22) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Immunohistochemistry - Anti-IkappaB-epsilon (phospho Ser22) Antibody (A93583)

Immunohistochemical analysis of paraffin-embedded human breast cancer using Anti-IkappaB-epsilon (phospho Ser22) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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