Anti-Ikaros Antibody [ARC0803] (A305301)

Name: Anti-Ikaros Antibody [ARC0803]
Brand: Antibodies.com
SKU: A305301
Price: 340.0 GBP
Availability: InStock

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$515

Rabbit monoclonal [ARC0803] antibody to Ikaros for WB, ICC/IF, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Ikaros Antibody [ARC0803]

Description

Rabbit monoclonal [ARC0803] antibody to Ikaros.

Applications

WB, ICC/IF, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, ChIP: 1:20-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Ikaros (Q13422).

Sequence

AEDLCKIGSERSLVLDRLASNVAKRKSSMPQKFLGDKGLSDTPYDSSASYEKENEMMKSHVMDQAINNAINYLGAESLRPLVQTPPGGSEVVPVISPMYQL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0803

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DNA-binding protein Ikaros, IK1, Ikaros family zinc finger protein 1, IKZF1, LYF1, Lymphoid transcription factor LyF-1, ZNFN1A1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Ikaros Antibody [ARC0803] (A305301)

Western blot analysis of extracts of various cell lines, using Anti-Ikaros Antibody (A305301) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunofluorescence - Anti-Ikaros Antibody [ARC0803] (A305301)

Immunofluorescence analysis of Jurkat cells using Anti-Ikaros Antibody (A305301) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Chromatin Immunoprecipitation - Anti-Ikaros Antibody [ARC0803] (A305301)

Chromatin immunoprecipitation (ChIP) analysis of extracts of K-562 cells, using Anti-Ikaros Antibody (A305301) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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