Western Blot - Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173)
Western blot analysis of extracts of various cell lines, using Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173) at 1:1,000 dilution. NIH/3T3 and C6 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.
Publishing research using Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173)
![Western Blot - Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306173_1.jpg?profile=product_top)
![Western Blot - Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306173_1.jpg?profile=product_top_thumb)
![Western Blot - Anti-IKB alpha (phospho Ser32) Antibody [ARC0147] (A306173) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306173_1.jpg?profile=product_image)
![Western Blot - Anti-IKB alpha (phospho Ser36) Antibody [ARC1543] (A306544) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306544_1.jpg?profile=product_alternative)
