Rabbit polyclonal antibody to IL-10R alpha (Phospho-Tyr496)
Specificity
The antibody detects endogenous level of IL-10R alpha only when phosphorylated at tyrosine 496.
Applications
WB
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Tyrosine496 (K-G-Y(p)-L-K) derived from Human IL-10R alpha.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts HUVEC tissue using IL-10R alpha (Phospho-Tyr496) antibody #11922.The lane on the right is treated with the antigen-specific peptide.
