This antibody recognizes human interleukin 17A (IL-17A; secreted or intracellular).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests Intracellular staining.
Reactivity
Human
Immunogen
Mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A.
Host
Mouse
Clonality
Monoclonal
Clone ID
9F9
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human CD17A positive CD3 positive lymphocytes (red-filled) from CD17A negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated and Brefeldin A treated human peripheral whole blood stained with Anti-IL-17A Antibody [9F9] (APC) (A320755), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with IL-17A
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.