This antibody is expected to cross react with isoforms NP_004624.1 and NP_001248348.1.
Immunogen
Synthetic peptide corresponding to the internal region of human IL-1R-2.
Sequence
C-QYNITRSIELRIKK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
45 kDa
Predicted MW
45.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CD121 antigen-like family member B, CD121b, CDw121b, IL-1 type II receptor, IL-1R-beta, IL-1RT-2, IL-1RT2, IL1R2, IL1RB, Interleukin-1 receptor beta, Interleukin-1 receptor type 2, Interleukin-1 receptor type II
IL-1R-2 expression in LNCaP (A) and MOLT4 (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-IL-1R-2 Antibody (A286042) at 0.5µg/ml (A) or 1µg/ml (B) and detected by chemiluminescence.
IL-1R-2 expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IL-1R-2 Antibody (A286042) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
IL-1R-2 expression in A549 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IL-1R-2 Antibody (A286042) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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