Anti-IL-2 Receptor alpha Antibody [9.14] (FITC) (A278702)

Anti-IL-2 Receptor alpha Antibody [9.14] (FITC)

Mouse monoclonal [9.14] antibody to IL-2 Receptor alpha (FITC).

This antibody recognises the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Ra), a single pass type I membrane protein expressed by activated ovine T-cells.Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132). A non-covalent association of the a and ß subunits is required to form the high affinity receptor for IL-2. Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Ra in other species including humans (Verhagen et. al.1993).Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011). Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1 x 10⁶ cells in 100µl

Sheep

Mouse

Monoclonal

9.14

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Sheep CD25, clone 9.14 recognizes the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Ra), a single pass type I membrane protein expressed by activated ovine T-cells.Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132). A non-covalent association of the a and ß subunits is required to form the high affinity receptor for IL-2.Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Ra in other species including humans (Verhagen et. al.1993).Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011).Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25.

This product is for research use only. It is not intended for diagnostic or therapeutic use.