Mouse monoclonal [MEM-181] antibody to IL-2 Receptor alpha (APC).
Specificity
This antibody reacts with an extracellular epitope of CD25, a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes / macrophages, and on CD4+ T lymphocytes (T regulatory cells). It is lost on resting B and T lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
PHA-activated peripheral blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-181
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Surface staining of PHA-stimulated (3 days) human PBMC with Anti-CD25 Antibody (A85771).
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