Anti-IL-2 Receptor alpha Antibody [OX-39] (PE) (A281447)

Anti-IL-2 Receptor alpha Antibody [OX-39] (PE)

Mouse monoclonal [OX-39] antibody to IL-2 Receptor alpha (PE).

This antibody recognises the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Stimulated Rat T cells.

Mouse

Monoclonal

OX-39

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987).

This product is for research use only. It is not intended for diagnostic or therapeutic use.