Anti-IL-2 Receptor alpha Antibody [P4A10] (A281543)

Anti-IL-2 Receptor alpha Antibody [P4A10]

Mouse monoclonal [P4A10] antibody to IL-2 Receptor alpha.

This antibody recognises the canine homologue of the human CD25 cell surface antigen, also known at IL-2Ra, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes. The IL-2 receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132), CD25 functions as a low affinity receptor for IL-2.Antibodies to CD4,Mouse anti Human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25. However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody. The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers , and in genetically related diseases of the hemopoietic system (Bauer Jr.et al. 2009).

WB, Flow Cytometry

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Canine

Enriched and stimulated Canine T cells.

Mouse

Monoclonal

P4A10

IgG1

1 mg/ml

Detects a double band of approximately 55 kDa in PMA-IO activated canine PBMC lysates.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Dog CD25, clone P4A10 recognizes the canine homologue of the human CD25 cell surface antigen, also known at IL-2Ra, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes (Abrams et al. 2010). The IL-2 receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132), CD25 functions as a low affinity receptor for IL-2.Antibodies to CD4,Mouse anti Human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25 (Rissetto et al. 2010). However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abrams et al. 2010).The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloni et al. 2008), and in genetically related diseases of the hemopoietic system (Bauer Jr.et al. 2009).