Rat monoclonal [PC61.5.3] antibody to IL-2 Receptor alpha (PE).
Specificity
This antibody recognises the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
B6.1 CTL cell line.
Host
Rat
Clonality
Monoclonal
Clone ID
PC61.5.3
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of ascites.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
