Anti-IL-25 Antibody [A14-3D2] (Biotin) (A279597)

Anti-IL-25 Antibody [A14-3D2] (Biotin)

Mouse monoclonal [A14-3D2] antibody to IL-25 (Biotin).

This antibody recognises interleukin-25 (IL-25) also known as IL17E. IL-25 is a member of the IL-17 family of cytokines and is expressed by various immune cells including mast cells, eosinophils, basophils and T helper type 2 cells. It is also expressed in non-hematopoietic cells such as epithelial and endothelial cells. As a pleiotropic cytokine, it stimulates stromal, innate immune and adaptive immune cells to regulate Th2 type inflammation. IL-25 exerts its biological function through its receptor IL-17RB, although there is some evidence that it may also bind to the receptor IL-17RA. Through this interaction, IL-25 is able to induce expression of IL-4, IL-5, IL-13 and TSLP. IL-25 is upregulated in asthmatic conditions and can induce typical eosinophil inflammation and airway hyperresponsiveness. IL-25 levels are also associated with the severity of chronic allergic reactions. The biotinylated Mouse anti Human interleukin-25 antibody, clone A14 -3D2 (MCA6249B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-25 antibody, clone A09 -3D10 (MCA6248GA) as the capture antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human full length interleukin-25 (Tyr33-Gly177).

Mouse

Monoclonal

A14-3D2

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human interleukin-25, clone A14-3D2, recognizes interleukin-25 (IL-25) also known as IL17E. IL-25 is a member of the IL-17 family of cytokines and is expressed by various immune cells including mast cells, eosinophils, basophils and T helper type 2 cells. It is also expressed in non-hematopoietic cells such as epithelial and endothelial cells (Yao et al. 2016). As a pleiotropic cytokine, it stimulates stromal, innate immune and adaptive immune cells to regulate Th2 type inflammation (Monin & Gaffen 2018). IL-25 exerts its biological function through its receptor IL-17RB, although there is some evidence that it may also bind to the receptor IL-17RA (Yao et al. 2016). Through this interaction, IL-25 is able to induce expression of IL-4, IL-5, IL-13 and TSLP (McGeachy et al. 2019). IL-25 is upregulated in asthmatic conditions and can induce typical eosinophil inflammation and airway hyperresponsiveness (Yao et al. 2016). IL-25 levels are also associated with the severity of chronic allergic reactions (Monin & Gaffen 2018). The biotinylated Mouse anti Human interleukin-25 antibody, clone A14 -3D2 (MCA6249B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-25 antibody, clone A09 -3D10 (MCA6248GA) as the capture antibody.

IL-17E, IL17E, IL25, Interleukin-17E, Interleukin-25

This product is for research use only. It is not intended for diagnostic or therapeutic use.