Anti-IL-31 Antibody [A12-2F7] (Biotin) (A279596)

Anti-IL-31 Antibody [A12-2F7] (Biotin)

Mouse monoclonal [A12-2F7] antibody to IL-31 (Biotin).

This antibody recognises interleukin-31 (IL-31), a member of the gp130/IL-6 family of proteins. It is a cytokine that has pro-inflammatory properties. IL-31 is mainly produced by CD4+ T helper and mast cells. IL-31 acts upon both immune and non-immune cells, regulating signaling of pro-inflammatory cytokines, cell proliferation and tissue remodeling. The effect of IL-31 is mediated by its receptor IL-31RA. Upon binding to this receptor, the JAK/STAT, PI3K/AKT and MAPK pathways can be activated. IL-31 has been implicated in chronic inflammation, with elevated levels detected in various skin diseases, Crohn’s disease and ulcerative colitis. Higher IL-31 levels in serum have also been positively correlated with asthma severity. The biotinylated Mouse anti Human interleukin-31 antibody, clone A12-2F7 (MCA6247B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-31 antibody, clone A01-1E4/A09.2-2E6 (MCA6246GA) as the capture antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human full length interleukin-31 (Ser24-Thr164).

Mouse

Monoclonal

A12-2F7

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human interleukin-31 antibody, clone A12-2F7, recognizes interleukin-31 (IL-31), a member of the gp130/IL-6 family of proteins. It is a cytokine that has pro-inflammatory properties. IL-31 is mainly produced by CD4+ T helper and mast cells (Di Salvo et al. 2018). IL-31 acts upon both immune and non-immune cells, regulating signaling of pro-inflammatory cytokines, cell proliferation and tissue remodeling. The effect of IL-31 is mediated by its receptor IL-31RA. Upon binding to this receptor, the JAK/STAT, PI3K/AKT and MAPK pathways can be activated (Zhang et al. 2008). IL-31 has been implicated in chronic inflammation, with elevated levels detected in various skin diseases, Crohn’s disease and ulcerative colitis. Higher IL-31 levels in serum have also been positively correlated with asthma severity (Lai et al. 2016).The biotinylated Mouse anti Human interleukin-31 antibody, clone A12-2F7 (MCA6247B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-31 antibody, clone A01-1E4/A09.2-2E6 (MCA6246GA) as the capture antibody.

IL31, Interleukin-31

This product is for research use only. It is not intended for diagnostic or therapeutic use.