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Anti-Influenza A Virus Nucleoprotein Antibody (A335)

Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HA Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses. Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.
$590
Product Datasheet

Mouse monoclonal (C43) antibody to Influenza A Virus Nucleoprotein for WB, IP, IF, IHC and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Influenza A Virus Nucleoprotein Antibody
Description
Mouse monoclonal (C43) antibody to Influenza A Virus Nucleoprotein.
Specificity
This antibody reacts with NP of all Influenza A viruses tested so far, including seasonal H2N2, H3N2, and avian H5N1, H5N2, and H1N1 (seasonal, pandemic, and swine).
Applications
WB, IP, IF, IHC, ELISA
Dilutions
WB: 1:300-1:1,000, IP: 1:100, IF: 1:200, IHC: 1:200
Reactivity
Influenza A Virus
Cross Reactivity
This antibody does not react with Influenza B viruses.
Immunogen
Human Influenza A Virus (H2N2) Okada strain.
Host
Mouse
Clonality
Monoclonal
Clone ID
C43
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Purity
> 90% (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. C43 antibody efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibodyEnlarge Image

Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody (A335)

Anti-Influenza A Virus Nucleoprotein Antibody
Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). C43 specifically recognized NP-expressing cells while a commercially available mouse anti-HA monoclonal antibody specifically recognized HAEnlarge Image

Immunofluorescence - Anti-Influenza A Virus Nucleoprotein Antibody (A335)

Anti-Influenza A Virus Nucleoprotein Antibody
Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 as a primary antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses.Enlarge Image

Anti-Influenza A Virus Nucleoprotein Antibody (A335)

Anti-Influenza A Virus Nucleoprotein Antibody
Identificationof Influenza Nucleoprotein in crude extract of MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34 using C43 mnoclonal antibody. 10-20% gradient gel Blotting 15v, 30min (semi-dry) blocking over night, 4? 1st antibody 1/1000 dilution 2nd antibody 1/10,000 dilution; rabbit polyclonal secodary antibody to mouse IgG- H & L (HRP) (ab97046; abcam). Positions of molecular size markers are shown in kDa on the left. NP size is 56 kDa according to Swiss-Prot.Enlarge Image

Anti-Influenza A Virus Nucleoprotein Antibody (A335)

Anti-Influenza A Virus Nucleoprotein Antibody
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