Mouse monoclonal [9F10] antibody to Integrin alpha 4/CD49D (APC-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD49d, a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
9F10
Isotype
IgG1
Light Chains
kappa
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
145-180 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD49d positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
![Flow Cytometry - Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (A122101) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122102_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (A122102) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122102_2.jpg?profile=product_top)
![Flow Cytometry - Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (A122101) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122102_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (A122102) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122102_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (A122101) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122102_1.jpg?profile=product_image)
![Flow Cytometry - Anti-CD49d Antibody [9F10] (APC-Cyanine 7) (A122102) - Antibodies.com](https://cdn.antibodies.com/image/catalog/122/A122102_2.jpg?profile=product_image)