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Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356)

Western Blot - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) - Antibodies.com Immunohistochemistry - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) - Antibodies.com Western Blot - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC0888] antibody to Integrin alpha 4/CD49D for WB, IHC and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Integrin alpha 4/CD49D Antibody [ARC0888]
Description
Rabbit monoclonal [ARC0888] antibody to Integrin alpha 4/CD49D.
Applications
WB, IHC, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 933-1032 of human Integrin alpha 4 (ITGA4/CD49d) (P13612).
Sequence
ALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0888
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
70 kDa / 140 kDa / 150 kDa / 200 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) - Antibodies.comEnlarge Image

Western Blot - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356)

Western blot analysis of extracts of various cell lines, using Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) - Antibodies.comEnlarge Image

Western Blot - Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356). This Western blot was performed on the immunoprecipitate using Anti-Integrin alpha 4/CD49D Antibody [ARC0888] (A305356) at a dilution of 1:500.
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