Anti-Integrin alpha 4 / CD49D Antibody [Bu49] (A279899)

Anti-Integrin alpha 4 / CD49D Antibody [Bu49]

Mouse monoclonal [Bu49] antibody to Integrin alpha 4 / CD49D.

This antibody recognises human CD49d, also known as integrin alpha 4 or VLA-4 alpha subunit. CD49d is a ~150 kDa single pass type I transmembrane glycoprotein. CD49d can be proteolytically cleaved to yield fragments of ~80 and ~70 kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria. CD49d is expressed on a broad range of cells including lymphocytes, monocytes, eosinophils and thymocytes. CD49d expression may serve as a prognostic parker of survival and disease progression in patients with chronic lymphocytic leukemia and may have therapeutic potential. Mouse anti human CD49, clone Bu49 cross-reacts with the canine B-cell leukemia cell line GL-1 and induces homotypic cell aggregation.

IHC-Fr, Flow Cytometry, IP

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human, Dog

Hyatt myeloma cell line.

Mouse

Monoclonal

Bu49

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD49d monoclonal antibody, clone Bu49 recognizes human CD49d, also known as integrin alpha 4 or VLA-4 alpha subunit. CD49d is a ~150 kDa single pass type I transmembrane glycoprotein. CD49d can be proteolytically cleaved to yield fragments of ~80 and ~70 kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). CD49d is expressed on a broad range of cells including lymphocytes, monocytes, eosinophils and thymocytes. CD49d expression may serve as a prognostic parker of survival and disease progression in patients with chronic lymphocytic leukemia and may have therapeutic potential (Bulian et al. 2008).Mouse anti human CD49, clone Bu49 cross-reacts with the canine B-cell leukemia cell line GL-1 and induces homotypic cell aggregation (Ikewaki et al. 2010).