Rat monoclonal [R1-2] antibody to Integrin alpha 4/CD49D (PE).
Specificity
This antibody recognizes an extracellular epitope of murine CD49d, an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Mouse
Immunogen
Murine T lymphoma line TK1.
Host
Rat
Clonality
Monoclonal
Clone ID
R1-2
Isotype
IgG2b
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
500 µg/ml
Predicted MW
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of murine CD49d positive cells (red-filled) from unstained sample (black-dashed) in flow cytometry surface staining of murine splenocyte suspension, labeling Integrin alpha 4/CD49D with Anti-Integrin alpha 4/CD49D Antibody [R1-2] (PE) (A121820), (concentration in sample 5 µg/ml).
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