Rabbit polyclonal antibody to Integrin b3 (Phospho-Tyr773)
Specificity
The antibody detects endogenous level of Integrin b3 only when phosphorylated at tyrosine 773.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from Human Integrin b3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from 293 cells using Integrin b3 (Phospho-Tyr773) Antibody #11060 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Integrin b3 (Phospho-Tyr773) Antibody #11060 (left) or the same antibody preincubated with blocking peptide (right).
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