Mouse monoclonal [4S.B3] antibody to Interferon gamma (Biotin).
Specificity
This antibody recognizes Interferon gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. This antibody binds both glycosylated and non-glycosylated Interferon gamma.
Applications
Flow Cytometry, IP, WB, IHC-P, ICC, ELISA
Dilutions
Flow Cytometry: 1-4 µg/ml; Intracellular staining, ELISA: Detection antibody in combination with capture antibody NIB42.
Reactivity
Human, Non-Human Primates
Immunogen
Interferon gamma derived from human leukocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
4S.B3
Isotype
IgG1
Light Chains
kappa
Conjugate
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
Predicted MW
16-25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Cytokine IFN gamma (IFN?) sandwich ELISA (enzyme-linked immunosorbent assay) that utilizes Anti-Interferon gamma Antibody [NIB42] (A86390) as the protein capture antibody and Anti-Interferon gamma Antibody [4S.B3] (Biotin) (A86394) as the detection antibody. Recombinant IFN? was captured by A86390 and detected by variable amounts of A86394. Anti-Interferon gamma Antibody [4S.B3] (Biotin) (A86394) was visualized by Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of cytokine and to the amount of the detection antibody.
Separation of human IFN-gamma positive CD3 positive lymphocytes (red-filled) from IFN-gamma negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood (PHA stimulated and Brefeldin A + Monesin treated) stained with Anti-Interferon gamma Antibody [4S.B3] (Biotin) (A86394), (concentration in sample 1,7 µg/ml, Streptavidin APC).
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