Anti-Interferon gamma Antibody [8C4H9] (A279540)

Anti-Interferon gamma Antibody [8C4H9]

Mouse monoclonal [8C4H9] antibody to Interferon gamma.

This antibody recognises interferon gamma (IFN gamma) also known as immune interferon, a type II interferon. It is a cytokine which is mainly expressed by CD4+ Th1 T lymphocytes and natural killer cells. IFN gamma plays many roles within immunity, including the activation of macrophages, T helper lymphocytes, stimulating antigen presentation, inflammation and leukocyte trafficking. After infection antigen presenting cells secrete IL-12, IL-18 and IL-1 beta which can promote IFN gamma synthesis. IFN gamma can then elicit responses through interferon gamma receptor 1 and interferon gamma receptor 2. Activation of these receptors can then trigger immune responses through the JAK/STAT signaling pathway. Under physiological conditions, IFN gamma is constitutively expressed through a tightly controlled mechanism. However, it has been shown to have some protumorigenic roles, by upregulating checkpoint inhibitor programmed cell-death ligand 1. Conversely though, there have been some positive associations between IFN gamma and patient survival in several cancer types. As such, the exact roles that IFN gamma plays in cancer may largely be dependent on tumor specific context and microenvironmental cues. The purified Mouse anti Human IFN gamma antibody, clone 8C4H9 (MCA6238GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human IFN gamma antibody, clone 18F11G6 (MCA6239B) as the detection antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human full length IFN gamma (Gln24-Gln166).

Mouse

Monoclonal

8C4H9

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human IFN gamma antibody, clone 8C4H9, recognizes interferon gamma (IFN gamma) also known as immune interferon, a type II interferon. It is a cytokine which is mainly expressed by CD4+ Th1 T lymphocytes and natural killer cells. IFN gamma plays many roles within immunity, including the activation of macrophages, T helper lymphocytes, stimulating antigen presentation, inflammation and leukocyte trafficking (Pereiro et al. 2019). After infection antigen presenting cells secrete IL-12, IL-18 and IL-1 beta which can promote IFN gamma synthesis (Schoenborn & Wilson 2007). IFN gamma can then elicit responses through interferon gamma receptor 1 and interferon gamma receptor 2. Activation of these receptors can then trigger immune responses through the JAK/STAT signaling pathway (Pereiro et al. 2019). Under physiological conditions, IFN gamma is constitutively expressed through a tightly controlled mechanism. However, it has been shown to have some protumorigenic roles, by upregulating checkpoint inhibitor programmed cell-death ligand 1. Conversely though, there have been some positive associations between IFN gamma and patient survival in several cancer types. As such, the exact roles that IFN gamma plays in cancer may largely be dependent on tumor specific context and microenvironmental cues (Castro et al. 2018).The purified Mouse anti Human IFN gamma antibody, clone 8C4H9 (MCA6238GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human IFN gamma antibody, clone 18F11G6 (MCA6239B) as the detection antibody.