Mouse monoclonal [Ds-2] antibody to Intra Acrosomal Protein.
Specificity
This antibody reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Applications
ICC, WB
Dilutions
IHC: Membrane permeabilization (acetone) is essential.
Reactivity
Canine
Immunogen
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Host
Mouse
Clonality
Monoclonal
Clone ID
Ds-2
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
42 kDa, 92 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Acrosomal protein SP 10, Acrosomal protein SP-10, Acrosomal vesicle protein 1, ACRV1, ASPX_HUMAN, D11S4365, Msa63, SP 10, Sp10, SPACA2, Sperm protein 10
