Western Blot - Anti-IRAK4 (phospho Ser346) Antibody [ARC64221] (A329540)
IRAK4 expression in Karpas 299 cell lysates analyzed by western blot. Cells were treated with IL-1ß (200 ng/ml) at 37°C for 15 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-IRAK4 (Phospho Ser346) Antibody [ARC64221] (A329540) at a dilution of 1:1000 and detected with chemiluminescence.
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