Rabbit polyclonal antibody to ITCH (Phospho-Tyr420)
Specificity
The antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody #11774 (left)or the same antibody preincubated with blocking peptide (right).
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