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Anti-ITK/EMT Antibody [ARC58362] (A309723)

Western Blot - Anti-ITK/EMT Antibody [ARC58362] (A309723) - Antibodies.com Western Blot - Anti-ITK/EMT Antibody [ARC58362] (A309723) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC58362] antibody to ITK/EMT for WB and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-ITK/EMT Antibody [ARC58362]
Description
Rabbit monoclonal [ARC58362] antibody to ITK/EMT.
Applications
WB, IP
Dilutions
WB: 1:2,000-1:6,000, IP: 1:2,000-1:6,000
Reactivity
Human
Immunogen
Recombinant protein of human ITK/EMT.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC58362
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
75 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-ITK/EMT Antibody [ARC58362] (A309723) - Antibodies.comEnlarge Image

Western Blot - Anti-ITK/EMT Antibody [ARC58362] (A309723)

Western blot analysis of various lysates, using Anti-ITK/EMT Antibody [ARC58362] (A309723) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-ITK/EMT Antibody [ARC58362] (A309723) - Antibodies.comEnlarge Image

Western Blot - Anti-ITK/EMT Antibody [ARC58362] (A309723)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-ITK/EMT Antibody [ARC58362] (A309723). This Western blot was performed on the immunoprecipitate using Anti-ITK/EMT Antibody [ARC58362] (A309723) at a dilution of 1:5000.
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