Western blot analysis of extracts of K-562 cells, using Anti-JAK2 Antibody (A81051) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using Anti-JAK2 Antibody (A81051) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-JAK2 Antibody (A81051) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-JAK2 Antibody (A81051) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-JAK2 Antibody (A81051) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-JAK2 Antibody (A81051) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using Anti-JAK2 Antibody (A81051) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 25µg extracts of mouse lung cells using 3µg of Anti-JAK2 Antibody (A81051). This Western blot was performed on the immunoprecipitate using Anti-JAK2 Antibody (A81051) at a dilution of 1:1000.
