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Anti-JNK1 + JNK2 + JNK3 Antibody (A307842)

Western Blot - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.com Immunohistochemistry - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.com Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.com Western Blot - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to JNK1 + JNK2 + JNK3 for WB, IHC, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-JNK1 + JNK2 + JNK3 Antibody
Description
Rabbit polyclonal antibody to JNK1 + JNK2 + JNK3.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3 (NP_620637.1).
Sequence
MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
46 kDa / 54 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.comEnlarge Image

Western Blot - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842)

Western blot analysis of extracts of various cell lines, using Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842)

Immunofluorescence analysis of U2OS cells using Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) - Antibodies.comEnlarge Image

Western Blot - Anti-JNK1 + JNK2 + JNK3 Antibody (A307842)

Immunoprecipitation analysis of 600µg extracts of mouse brain cells using 3µg of Anti-JNK1 + JNK2 + JNK3 Antibody (A307842). This Western blot was performed on the immunoprecipitate using Anti-JNK1 + JNK2 + JNK3 Antibody (A307842) at a dilution of 1:1000.
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