The antibody detects endogenous level of JNK1 / JNK2 / JNK3 only when phosphorylated at Thr183 / Tyr185.
Applications
WB, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Thr183 / Tyr185 (M-M-T(p)-P-Y(p)- V - V ) derived from Human JNK1 / JNK2 / JNK3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
46 54 kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
c-Jun N-terminal kinase 1, JNK-46, MAP kinase 8, MAPK 8, MAPK8, Mitogen-activated protein kinase 8, PRKM8, SAPK1, SAPK1C, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1
Western blot analysis of extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody #11504.
Alternative products to Anti-JNK1 / JNK2 / JNK3 (phospho-Thr183 / Tyr185) Antibody (A50524)
