Anti-K48-linkage specific Polyubiquitin Antibody (A309530)

Name: Anti-K48-linkage specific Polyubiquitin Antibody
Brand: Antibodies.com
SKU: A309530

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This product is discontinued

Anti-K48-linkage specific Polyubiquitin Antibody (A309530) has been discontinued and is no longer available.

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Specifications

Name

Anti-K48-linkage specific Polyubiquitin Antibody

Description

Rabbit polyclonal antibody to K48-linkage specific Polyubiquitin.

Applications

WB, Dot Blot

Dilutions

WB: 1:100-1:500, DB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K48 linkage).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 - 250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-K48-linkage specific Polyubiquitin Antibody (A309530)

Western blot analysis of extracts of various cell lines, using Anti-K48-linkage specific Polyubiquitin Antibody (A309530) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.