KAISO expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-KAISO Antibody (A87683) at a dilution of 1:3000 and detected with chemiluminescence.
Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-KAISO Antibody (A87683) and Rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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