Anti-KCNQ1 Antibody [N37A/10] (A305125)

Name: Anti-KCNQ1 Antibody [N37A/10]
Brand: Antibodies.com
SKU: A305125
Price: 390.0 GBP
Availability: InStock

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$520

Mouse monoclonal [N37A/10] antibody to KCNQ1 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-KCNQ1 Antibody [N37A/10]

Description

Mouse monoclonal [N37A/10] antibody to KCNQ1.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Hamster

Immunogen

Fusion protein amino acids 2-101 of human KCNQ1.

Host

Mouse

Clonality

Monoclonal

Clone ID

N37A/10

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was formely sold under the Clone ID S37A-10.

Synonyms

IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1, KCNA8, KCNA9, KQT-like 1, KVLQT1, Potassium voltage-gated channel subfamily KQT member 1, Voltage-gated potassium channel subunit Kv7.1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Immunofluorescence - Anti-KCNQ1 Antibody [N37A/10] (A305125)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-KCNQ1 Antibody [N37A/10] (A305125), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCNQ1 Antibody (D) Composite.

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Western Blot - Anti-KCNQ1 Antibody [N37A/10] (A305125)

Western blot analysis of Hamster T-CHO cell lysate showing detection of KCNQ1 protein using Anti-KCNQ1 Antibody [N37A/10] (A305125) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Immunohistochemistry - Anti-KCNQ1 Antibody [N37A/10] (A305125)

Immunohistochemistry analysis of mouse brain slice, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-KCNQ1 Antibody [N37A/10] (A305125) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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Western Blot - Anti-KCNQ1 Antibody [N37A/10] (A305125)

Western blot analysis of human Cell lysates showing detection of KCNQ1 protein using Anti-KCNQ1 Antibody [N37A/10] (A305125) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.