Anti-KDEL Antibody [2C1] (A305072)

Name: Anti-KDEL Antibody [2C1]
Brand: Antibodies.com
SKU: A305072
Price: 390.0 GBP
Availability: InStock

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$520

Mouse monoclonal [2C1] antibody to KDEL for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

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Specifications

Name

Anti-KDEL Antibody [2C1]

Description

Mouse monoclonal [2C1] antibody to KDEL.

Specificity

This antibody detects KDEL tagged proteins.

Applications

WB, IHC

Dilutions

WB: 1:1,000, IHC: 1:25

Reactivity

Human, Mouse, Rat

Immunogen

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA).

Host

Mouse

Clonality

Monoclonal

Clone ID

2C1

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-KDEL Antibody [2C1] (A305072)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Anti-KDEL Antibody [2C1] (A305072) at 1:1,000 for 2 hours at room temperature.. Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Mouse IgG:HRP at 1:5,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: Multiple.

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Immunohistochemistry - Anti-KDEL Antibody [2C1] (A305072)

Immunohistochemistry analysis of rat kidney, fixed in formalin. The Primary Antibody used was Anti-KDEL Antibody [2C1] (A305072) at 1:25 for 1 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.

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