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Anti-KDM3B/JMJD1B Antibody (A11529)

Western Blot - Anti-KDM3B/JMJD1B Antibody (A11529) - Antibodies.com Immunohistochemistry - Anti-KDM3B/JMJD1B Antibody (A11529) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to KDM3B/JMJD1B for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-KDM3B/JMJD1B Antibody
Description
Rabbit polyclonal antibody to KDM3B/JMJD1B.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human KDM3B (NP_057688.2).
Sequence
GAENKEAGKTLEQVGQGIVASAAVVTTASSTPNTVRISDTGLAAGTVPEKQKGSRSQASGENSRNSILASSGFGAPLPSSSQPLTFGSGRSQSNGVLATENKPLGFSFGCSSAQEAQKDTDLSKNLFFQCMSQTLPTSNYFTTVSESLADDSSSRDSFKQSLESLSSGLCKGRSVLGTDTKPGSKAGSSVDRKVPAESMPT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
225 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-KDM3B/JMJD1B Antibody (A11529) - Antibodies.comEnlarge Image

Western Blot - Anti-KDM3B/JMJD1B Antibody (A11529)

Western blot analysis of extracts of HeLa cells, using Anti-KDM3B/JMJD1B Antibody (A11529) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-KDM3B/JMJD1B Antibody (A11529) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-KDM3B/JMJD1B Antibody (A11529)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-KDM3B/JMJD1B Antibody (A11529) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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