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Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212)

Western Blot - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.com Immunofluorescence - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.com Immunofluorescence - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.com Western Blot - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.com Chromatin Immunoprecipitation - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1416] antibody to KDM4B/JMJD2B for WB, ICC/IF, IP and ChIP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-KDM4B/JMJD2B Antibody [ARC1416]
Description
Rabbit monoclonal [ARC1416] antibody to KDM4B/JMJD2B.
Applications
WB, ICC/IF, IP, ChIP
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KDM4B/JMJD2B (O94953).
Sequence
PPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWN
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1416
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western Blot - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.comEnlarge Image

Western Blot - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212)

Western blot analysis of extracts of various cell lines, using Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212)

Immunofluorescence analysis of C6 cells using Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212)

Immunofluorescence analysis of U-2 OS cells using Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) - Antibodies.comEnlarge Image

Western Blot - Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212)

Immunoprecipitation analysis of 300µg extracts of HCT116 cells using 3µg of Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212). This Western blot was performed on the immunoprecipitate using Anti-KDM4B/JMJD2B Antibody [ARC1416] (A308212) at a dilution of 1:1000.
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