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Anti-KDM5B/PLU1/Jarid1B Antibody (A10219)

Western Blot - Anti-KDM5B/PLU1/Jarid1B Antibody (A10219) - Antibodies.com Immunofluorescence - Anti-KDM5B/PLU1/Jarid1B Antibody (A10219) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to KDM5B/PLU1/Jarid1B for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-KDM5B/PLU1/Jarid1B Antibody
Description
Rabbit polyclonal antibody to KDM5B/PLU1/Jarid1B.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 784-883 of human KDM5B (NP_006609.3).
Sequence
EESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQNQLTVNELRQFVTQLYALPCVLSQTPLLKDLLNRVEDFQQHSQKLLSEE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
176 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-KDM5B/PLU1/Jarid1B Antibody (A10219) - Antibodies.comEnlarge Image

Western Blot - Anti-KDM5B/PLU1/Jarid1B Antibody (A10219)

Western blot analysis of extracts from normal (control) and KDM5B knockout (KO) 293T cells, using Anti-KDM5B/PLU1/Jarid1B Antibody (A10219) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-KDM5B/PLU1/Jarid1B Antibody (A10219) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-KDM5B/PLU1/Jarid1B Antibody (A10219)

Immunofluorescence analysis of L929 using Anti-KDM5B/PLU1/Jarid1B Antibody (A10219) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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