Keap1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Keap1 Antibody (A13680) at a dilution of 1:500 and detected with chemiluminescence.
Keap1 expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Keap1 Antibody (A13680) at a dilution of 1:50.
Keap1 expression in human esophageal cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Keap1 Antibody (A13680) at a dilution of 1:50.
Keap1 expression in PC-12 cells analyzed by immunofluorescence. Staining was performed with Anti-Keap1 Antibody (A13680) at a dilution of 1:50 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
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