Recombinant Anti-Keratin 76 + Keratin 77 Antibody [4999R] - BSA and Azide free (A316595)

Anti-Keratin 76 + Keratin 77 Antibody [4999R] - BSA and Azide free

Recombinant rabbit oligoclonal [4999R] antibody to Keratin 76 + Keratin 77.

MonoPoly antibodies are designed by pooling several monospecific, recombinant monoclonal antibodies against a target. MonoPoly antibodies are a kind of synthetic polyclonal antibodies that can be produced in unlimited quantity with a strict lot-to-lot consistency. These antibodies are highly specific with exquisite sensitivity and a single MonoPoly antibody can be used for a variety of 45kDa (CK18), 40kDa (CK19) and 46kDa (CK20). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. This antibody is a broad-spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms.

Flow Cytometry, IF, WB, IHC-P

HeLa or MCF-7 cells. Human skin, adeno- or squamous carcinomas.

Flow Cytometry: 0.5-1 µg/million cells, IF: 1-2 µg/ml, WB: 0.5-2 µg/ml for 2 hours at RT, IHC: 1-2 µg/ml

Human

Recombinant fragments and/or synthetic peptides of human Cytokeratin proteins (exact sequences are proprietary).

Rabbit

Oligoclonal

IgG

kappa

Protein A/G chromatography.

1 mg/ml

40-67 kDa (Multiple)

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.