Rabbit polyclonal antibody to KIF11 (phospho Thr926).
Specificity
This antibody detects endogenous levels of KIF11/Eg5 only when phosphorylated at Thr926.
Applications
WB, ELISA
Dilutions
IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:1000
Reactivity
Human
Immunogen
Synthetic peptide derived from human KIF11/Eg5 around the phosphorylation site of Thr926 (amino acids 892-941).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
119kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
KIF11, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, KNSL1, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, TRIP5
Western Blot - Anti-KIF11 (phospho Thr926) Antibody (A95653)
Western blot analysis of lysates from COLO205 cells using Anti-KIF11 (phospho Thr926) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human lung cancer using Anti-KIF11 (phospho Thr926) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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