Rabbit polyclonal antibody to Kir6.2 (Phospho-Thr224)
Specificity
The antibody detects endogenous levels of Kir6.2 only when phosphorylated at threonine 224.
Applications
WB, IF
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 224 (K-T-TP-S-P) derived from Human Kir6.2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HeLa cells, using Kir6.2 (Phospho-Thr224) antibody #12108. The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using Kir6.2 (Phospho-Thr224) antibody #12108. The picture on the right is treated with the synthesized peptide.
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