Synthetic peptide within amino acids 1000-1050 of human KMT4/Dot1L (NP_115871. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
200 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Chromatin from KOPN-8 cell line was immunoprecipitated with Anti-KMT4 / Dot1L Antibody (A295971) and analyzed by DNA sequencing. The figure illustrates the peak distribution of |KMT4 / DOT1L binding within a 250 Kb region of chromosome 10 as detected using Anti-KMT4 / Dot1L Antibody (A295971). ChIP-seq validation performed by Active Motif, Carlsbad, CA.
Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Anti-KMT4 / Dot1L Antibody (A295971) was used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. KMT4 / DOT1L was also immunoprecipitated by another rabbit anti-KMT4 / DOT1L antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Anti-KMT4 / Dot1L Antibody (A295971) was used for at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 3 minutes.
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