Anti-LAR Antibody [S165-38] (A305027)

Name: Anti-LAR Antibody [S165-38]
Brand: Antibodies.com
SKU: A305027
Price: 390.0 GBP
Availability: InStock

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$520

Mouse monoclonal [S165-38] antibody to LAR for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-LAR Antibody [S165-38]

Description

Mouse monoclonal [S165-38] antibody to LAR.

Specificity

Full length protein is 210 kDa band is smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK

Host

Mouse

Clonality

Monoclonal

Clone ID

S165-38

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Leukocyte common antigen related, PTPRF, Receptor-type tyrosine-protein phosphatase F

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-LAR Antibody [S165-38] (A305027)

Western blot analysis of rat brain membrane showing detection of LAR protein using Anti-LAR Antibody [S165-38] (A305027) at 1:250.

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Immunofluorescence - Anti-LAR Antibody [S165-38] (A305027)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-LAR Antibody [S165-38] (A305027), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LAR/PTPRF Antibody. (D) Composite.

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Immunofluorescence - Anti-LAR Antibody [S165-38] (A305027)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-LAR Antibody [S165-38] (A305027), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LAR/PTPRF Antibody (D) Composite.

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