Western Blot - Anti-LATS1/WARTS (phospho Ser909) + LATS2 (phospho Ser872) Antibody (A91496)
Western blot analysis of HeLa, using Anti-LATS1/WARTS (phospho Ser909) + LATS2 (phospho Ser872) Antibody (A91496) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
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