Anti-LC3B Antibody (A87552)

Name: Anti-LC3B Antibody
Brand: Antibodies.com
SKU: A87552
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to LC3B for WB, IHC-P and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Autophagosome Marker

Specifications

Name

Anti-LC3B Antibody

Description

Rabbit polyclonal antibody to LC3B.

Applications

WB, IHC-P, ELISA

Positive Control

HeLa, NIH/3T3, NIH/3T3, HeLa.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A recombinant fusion protein corresponding to amino acids 1-125 of human LC3B.

Sequence

MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

14 kDa, 16 kDa / 14 kDa / 16 kDa

Predicted MW

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-LC3B Antibody (A87552)

LC3B expression in NIH3T3 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 30ug protein per lane with Anti-LC3B Antibody (A87552) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-LC3B Antibody (A87552)

LC3B expression in HeLa cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 30ug protein per lane with Anti-LC3B Antibody (A87552) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-LC3B Antibody (A87552)

LC3B expression in human breast cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-LC3B Antibody (A87552) at a dilution of 1:100.

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Immunohistochemistry - Anti-LC3B Antibody (A87552)

LC3B expression in human gastric cancer tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-LC3B Antibody (A87552) at a dilution of 1:100.