Anti-Lck (phospho Tyr505) Antibody (A12479)

Western blot analysis of extracts of Jurkat cells, using Anti-Lck (phospho Tyr505) Antibody (A12479) at 1:1,000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. Jurkat cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 1s.

Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Lck (phospho Tyr505) Antibody (A12479). This Western blot was performed on the immunoprecipitate using Anti-Lck (phospho Tyr505) Antibody (A12479) at a dilution of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight.