This antibody recognizes an extracellular epitope of CD118, a transmembrane glycoprotein, which associates with CD130 to form the functional high affinity LIF receptor.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 4-10 µg/ml.
Reactivity
Human
Immunogen
Human recombinant soluble CD118-Fc.
Host
Mouse
Clonality
Monoclonal
Clone ID
12D3
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of JAR cells stained with Anti-LIFR Antibody [12D3] (PE) (A320771), (concentration in sample 15 µg/ml, red-filled) from JAR cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 15 µg/ml, same as CD118 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of JAR cell suspension.
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Proteins predicted to interact with LIFR
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-LIFR Antibody [12D3] (PE) (A320771)](https://cdn.antibodies.com/image/catalog/320/A320771_1.jpg?profile=product_top)
![Flow Cytometry - Anti-LIFR Antibody [12D3] (PE) (A320771)](https://cdn.antibodies.com/image/catalog/320/A320771_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-LIFR Antibody [12D3] (PE) (A320771)](https://cdn.antibodies.com/image/catalog/320/A320771_1.jpg?profile=product_image)