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Anti-LIG3 Antibody (A13724)

Western Blot - Anti-LIG3 Antibody (A13724) - Antibodies.com Immunoprecipitation - Anti-LIG3 Antibody (A13724) - Antibodies.com
$355

Rabbit polyclonal antibody to LIG3 for WB, IP and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-LIG3 Antibody
Description
Rabbit polyclonal antibody to LIG3.
Applications
WB, IP, ELISA
Positive Control
BT474, SW480, HeLa, HT-29, Jurkat.
Dilutions
WB: 1:500-1:2,000, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells
Reactivity
Human, Mouse
Immunogen
A recombinant fusion protein corresponding to amino acids 730-1009 of human LIG3.
Sequence
AGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
113 kDa
Predicted MW
113 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
D11Wsu78e, DNA ligase, DNA ligase 3, DNA Ligase III, DNLI3_HUMAN, LIG 3, LIG2, Ligase II, DNA, ATP dependent, Ligase III DNA ATP dependent, MGC78176, OTTMUSP00000000875, Polydeoxyribonucleotide synthase, Polydeoxyribonucleotide synthase [ATP], Polydeoxyribonucleotide synthase [ATP] 3, RP23-316C11.6
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-LIG3 Antibody (A13724) - Antibodies.comEnlarge Image

Western Blot - Anti-LIG3 Antibody (A13724)

LIG3 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-LIG3 Antibody (A13724) at a dilution of 1:1000 and detected with chemiluminescence.
Immunoprecipitation - Anti-LIG3 Antibody (A13724) - Antibodies.comEnlarge Image

Immunoprecipitation - Anti-LIG3 Antibody (A13724)

LIG3 expression in 200 µg extracts of Jurkat cells analyzed by western blot following immunoprecipitation, both using Anti-LIG3 Antibody (A13724) at 1 µg and a dilution of 1:1000, respectively.
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