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Anti-LIGHT/TNFSF14 Antibody (A82879)

Western Blot - Anti-LIGHT/TNFSF14 Antibody (A82879) - Antibodies.com Immunofluorescence - Anti-LIGHT/TNFSF14 Antibody (A82879) - Antibodies.com
$495
Product Datasheet

Goat polyclonal antibody to LIGHT/TNFSF14 for ELISA, WB and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-LIGHT/TNFSF14 Antibody
Description
Goat polyclonal antibody to LIGHT/TNFSF14.
Applications
ELISA, WB, IF
Dilutions
ELISA: 1:64,000, WB: 0.03-0.1µg/ml, IF: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human LIGHT/TNFSF14.
Sequence
C-HEVNPAAHLTGANS
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
20-22 kDa
Predicted MW
22.4kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-LIGHT/TNFSF14 Antibody (A82879) - Antibodies.comEnlarge Image

Western Blot - Anti-LIGHT/TNFSF14 Antibody (A82879)

LIGHT/TNFSF14 expression in Human Cerebellum (A) and Frontal Cortex (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-LIGHT/TNFSF14 Antibody (A82879) at 0.03µg/ml and detected by chemiluminescence.
Immunofluorescence - Anti-LIGHT/TNFSF14 Antibody (A82879) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-LIGHT/TNFSF14 Antibody (A82879)

LIGHT/TNFSF14 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-LIGHT/TNFSF14 Antibody (A82879) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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